Open AccessCollagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury
Author(s) -
Mu Hua,
Wang Yeqing,
Wei Haiying,
Lu Hong,
Feng Zhuolei,
Yu Hongmin,
Xing Yue,
Wang Haijing
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13768
Subject(s) - retinal , oxidative stress , antioxidant , apoptosis , reperfusion injury , drug , pharmacology , reactive oxygen species , chemistry , viability assay , drug delivery , ischemia , medicine , biochemistry , organic chemistry
Oxidative stress can cause injury in retinal endothelial cells. Carboxymethyl cellulose modified with collagen peptide ( CMCC ) is of a distinct antioxidant capacity and potentially a good drug carrier. In this study, the protective effects of CMCC against H 2 O 2 ‐induced injury of primary retinal endothelial cells were investigated. In vitro, we demonstrated that CMCC significantly promoted viability of H 2 O 2 ‐treated cells, efficiently restrained cellular reactive oxygen species ( ROS ) production and cell apoptosis. Then, the CMCC was employed as both drug and anti‐inflammatory drug carrier for treatment of retinal ischaemia/reperfusion (I/R) in rats. Animals were treated with CMCC or interleukin‐10‐loaded CMCC ( IL ‐10@ CMCC ), respectively. In comparisons, the IL ‐10@ CMCC treatment exhibited superior therapeutic effects, including better restoration of retinal structural thickness and less retinal apoptosis. Also, chemiluminescence demonstrated that transplantation of IL ‐10@ CMCC markedly reduced the retinal oxidative stress level compared with CMCC alone and potently recovered the activities of typical antioxidant enzymes, SOD and CAT . Therefore, it could be concluded that CMCC provides a promising platform to enhance the drug‐based therapy for I/R‐related retinal injury.