Open AccessLycium barbarum polysaccharides restore adverse structural remodelling and cardiac contractile dysfunction induced by overexpression of micro RNA ‐1
Author(s) -
Zhang Rong,
Xu Yi,
Niu Huifang,
Tao Ting,
Ban Tao,
Zheng Linyao,
Ai Jing
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13740
Subject(s) - lycium , heart failure , rna , microrna , microbiology and biotechnology , phosphorylation , adverse effect , transgene , messenger rna , medicine , chemistry , cardiology , cancer research , biology , gene , pathology , biochemistry , alternative medicine
Micro RNA ‐1 (miR‐1) stands out as the most prominent micro RNA (mi RNA ) in regulating cardiac function and has been perceived as a new potential therapeutic target. Lycium barbarum polysaccharides ( LBP s) are major active constituents of the traditional Chinese medicine based on L. barbarum . The purpose of this study was to exploit the cardioprotective effect and molecular mechanism of LBP s underlying heart failure. We found that LBP s significantly reduced the expression of myocardial miR‐1. LBP s improved the abnormal ECG and indexes of cardiac functions in P‐V loop detection in transgenic (Tg) mice with miR‐1 overexpression. LBP s recovered morphological changes in sarcomeric assembly, intercalated disc and gap junction. LBP s reversed the reductions of CaM and cMLCK , the proteins targeted by miR‐1. Similar trends were also obtained in their downstream effectors including the phosphorylation of MLC 2v and both total level and phosphorylation of Ca MKII and cMyBP ‐C. Collectively, LBP s restored adverse structural remodelling and improved cardiac contractile dysfunction induced by overexpression of miR‐1. One of the plausible mechanisms was that LBP s down‐regulated miR‐1 expression and consequently reversed miR‐1‐induced repression of target proteins relevant to myocardial contractibility. LBP s could serve as a new, at least a very useful adjunctive, candidate for prevention and therapy of heart failure.