Open AccessCombination of resveratrol and 5‐azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells
Author(s) -
Marycz Krzysztof,
Kornicka Katarzyna,
IrwinHouston Jennifer M.,
Weiss Christine
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13731
Subject(s) - mesenchymal stem cell , adipose tissue , microbiology and biotechnology , stem cell , resveratrol , autophagy , biology , cellular differentiation , chemistry , endocrinology , medicine , pharmacology , biochemistry , apoptosis , gene
Endocrine disorders have become more and more frequently diagnosed in humans and animals. In horses, equine metabolic syndrome ( EMS ) is characterized by insulin resistance, hyperleptinemia, hyperinsulinemia, inflammation and usually by pathological obesity. Due to an increased inflammatory response in the adipose tissue, cytophysiological properties of adipose derived stem cells ( ASC ) have been impaired, which strongly limits their therapeutic potential. Excessive accumulation of reactive oxygen species, mitochondria deterioration and accelerated ageing of those cells affect their multipotency and restrict the effectiveness of the differentiation process. In the present study, we have treated ASC isolated from EMS individuals with a combination of 5‐azacytydine ( AZA ) and resveratrol ( RES ) in order to reverse their aged phenotype and enhance osteogenic differentiation. Using SEM and confocal microscope, cell morphology, matrix mineralization and mitochondrial dynamics were assessed. Furthermore, we investigated the expression of osteogenic‐related genes with RT ‐ PCR . We also investigated the role of autophagy during differentiation and silenced PARKIN expression with si RNA . Obtained results indicated that AZA / RES significantly enhanced early osteogenesis of ASC derived from EMS animals. Increased matrix mineralization, RUNX ‐2, collagen type I and osteopontin levels were noted. Furthermore, we proved that AZA / RES exerts its beneficial effects by modulating autophagy and mitochondrial dynamics through PARKIN and RUNX ‐2 activity.