Open AccessCharacterization of induced pluripotent stem cell‐derived megakaryocyte lysates for potential regenerative applications
Author(s) -
Baigger Anja,
Eicke Dorothee,
Yuzefovych Yuliia,
Pogozhykh Denys,
Blasczyk Rainer,
Figueiredo Constanca
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13698
Subject(s) - growth factor , microbiology and biotechnology , induced pluripotent stem cell , epidermal growth factor , platelet , biology , platelet derived growth factor receptor , regenerative medicine , stem cell , megakaryocyte , stem cell factor , transforming growth factor , vascular endothelial growth factor , immunology , cell culture , cancer research , haematopoiesis , biochemistry , vegf receptors , embryonic stem cell , genetics , receptor , gene
Abstract Recently, platelet‐derived growth factors present in lysates became an extreme interest in the field of regenerative medicine such as in wound healing and as substitutes to foetal bovine serum in xeno‐free cell culture systems. However, the generation of such platelet lysates completely depends on the availability of platelet donors. In this study, the possibility to use in vitro ‐generated megakaryocytes derived from induced pluripotent stem cells ( iPSC s) as a cell source for typical platelet growth factors was investigated. Therefore, the presence and levels of those factors were characterized in in vitro ‐produced megakaryocytes. In comparison with platelets, in vitro ‐generated megakaryocytes showed a multifold increased content in transcript and protein levels of typical platelet growth factors including platelet‐derived growth factors ( PDGF s), transforming growth factor ( TGF )‐1β, vascular endothelial cell factor ( VEGF )‐A, epidermal growth factor ( EGF ), insulin‐like growth factor ( IGF )‐1 and tissue factor ( TF ). Hence, iPSC ‐derived megakaryocytes may serve as an efficient cell source for a donor‐independent generation of growth factor‐rich lysates with a broad application potential in innovative cell culture systems and regenerative therapies.