Open AccessAssociation between polymorphisms in the promoter region of miR‐17‐92 cluster and systemic lupus erythematosus in a Chinese population
Author(s) -
Wang Rong,
Wang ChunFang,
Qin HaiMei,
Lu YuLan,
Wei GuiJiang,
Huang HuaTuo,
Xiang Yang,
Wang JunLi,
Lan Yan,
Wei YeSheng
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13672
Subject(s) - haplotype , genotype , medicine , gastroenterology , allele , immunology , biology , gene , genetics
The aim of this study was to investigate the association of genetic polymorphisms in the promoter region of miR‐17‐92 with systemic lupus erythematosus ( SLE ). The gene polymorphism was analysed using SN aPshot in 312 SLE patients and 396 controls. Relative expression of miR‐17‐92 was measured by quantitative real‐time PCR . Association was found between rs9515692 and a decreased risk of SLE ( CT vs CC : OR = 0.65, 95% CI , 0.46‐0.92, P = .014; CT + TT vs CC : OR = 0.64, 95% CI , 0.46‐0.90, P = .009; T vs C: OR = 0.69, 95% CI , 0.52‐0.92, P = .010, respectively). Haplotype analysis showed that C‐G‐G, C‐A‐A haplotypes were associated with an increased SLE risk (OR=4.46, 95%CI, 2.17‐9.17, P < 0.001; OR=2.33, 95%CI, 1.44‐3.76, P < 0.001, respectively). T allele and CT + TT genotypes in rs9515692 were associated with decreased risk of anti‐ds DNA in SLE ( CT + TT vs CC : OR = 0.42, 95% CI = 0.24‐0.72, P = .002; T vs A: OR = 0.49, 95% CI = 0.31‐0.79, P = .003). Moreover, rs9515692 CT + TT genotypes had a higher level of miR‐17 as compared to CC genotype ( P = .017). These findings suggest that the rs9515692 CT + TT genotypes were a protective factor for the susceptibility of SLE , probably by increasing the expression of miR‐17.