Open AccessThe miR‐3127‐5p/p‐ STAT 3 axis up‐regulates PD ‐L1 inducing chemoresistance in non‐small‐cell lung cancer
Author(s) -
Tang Dongfang,
Zhao Dandan,
Wu Yun,
Yao Ruyong,
Zhou Lin,
Lu Liming,
Gao Wen,
Sun Yifeng
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13657
Subject(s) - stat , cancer research , biology , microbiology and biotechnology , signal transduction , stat3
It is less known about mi RNA 3127‐5p induced up‐regulation of PD ‐L1, immune escape and drug resistance caused by increased PD ‐L1 in lung cancer. In this study, lentivirus was transduced into lung cancer cells, and quantitative PCR and Western blot were used to detect the expression of PD ‐L1. Then immunofluorescence assay was applied to detect autophagy, finally we explored the relationship between PD ‐L1 expressions and chemoresistance in patients. As a result, we found that micro RNA ‐3127‐5p promotes pSTAT 3 to induce the expression of PD ‐L1; micro RNA ‐3127‐5p promotes STAT 3 phosphorylation through suppressing autophagy, and autophagy could retaine pSTAT 3 into the nucleus in mi RNA ‐3127‐5p knocked cells, and immune escape induced by elevated level of PD ‐L1 results in chemoresistance of lung cancer. In conclusion, micro RNA ‐3127‐5p induces PD ‐L1 elevation through regulating pSTAT 3 expression. We also demonstrate that immune escape induced by PD ‐L1 can be dismissed by corresponding monoclonal antibody.