Open AccessHuman umbilical cord mesenchymal stem cells facilitate the up‐regulation of miR‐153‐3p, whereby attenuating MGO ‐induced peritoneal fibrosis in rats
Author(s) -
Li Dong,
Lu Zhenyu,
Li Xiyuan,
Xu Zhongwei,
Jiang Jianqing,
Zheng Zhenfeng,
Jia Junya,
Lin Shan,
Yan Tiekun
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13622
Subject(s) - snai1 , mesenchymal stem cell , luciferase , western blot , microrna , mesothelial cell , epithelial–mesenchymal transition , transfection , cd90 , umbilical cord , chemistry , microbiology and biotechnology , andrology , downregulation and upregulation , medicine , biology , immunology , pathology , stem cell , gene , biochemistry , cd34
Mi RNA s contribute greatly to epithelial to mesenchymal transition ( EMT ) of peritoneal mesothelial cells ( PMC s), which is a crucial step in peritoneal fibrosis ( PF ). In this study, we tried to profile whether mi RNA expression differences exist after human umbilical cord mesenchymal stem cells ( hUCMSC s) treatment in PF rats and investigate the possible role of miR‐153‐3p involved in anti‐ EMT process. We randomly assigned 34 rats into three groups: control group (Group Control), MGO ‐induced PF rats (Group MGO ) and hUCMSC s‐treated rats (Group MGO + hUCMSC s). Mi RNA microarrays and real‐time PCR analyses were conducted in three groups. α‐ SMA , Snail1 and E‐cadherin expression were detected by Western blot. Luciferase reporter assays were used to detect the effects of miR‐153‐3p overexpression on Snai1 in rat peritoneal mesothelial cells ( RPMC s). We identified differentially expressed mi RNA s related to EMT , in which miR‐153‐3p demonstrated the greatest increase in Group MGO + hUCMSC s. Transient cotransfection of miR‐153‐3p mimics with luciferase expression plasmids resulted in a significant repression of Snai1 3′‐untranslated region luciferase activity in RPMC s. These studies suggest that miR‐153‐3p is a critical molecule in anti‐ EMT effects of hUCMSC s in MGO ‐induced PF rats. MiR‐153‐3p might exert its beneficial effect through directly targeting Snai1.