Open AccessUp‐regulation of miR‐10b‐3p promotes the progression of hepatocellular carcinoma cells via targeting CMTM 5
Author(s) -
Guan Lianyue,
Ji Degang,
Liang Na,
Li Shuo,
Sun Baozhen
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13620
Subject(s) - in vivo , reporter gene , cancer research , microrna , hepatocellular carcinoma , mtt assay , carcinogenesis , western blot , gentamicin protection assay , cell growth , luciferase , biology , cell culture , microbiology and biotechnology , gene expression , gene , transfection , genetics
In this study, we investigated how miR‐10b‐3p regulated the proliferation, migration, invasion in hepatocellular carcinoma ( HCC ) at both in vitro and in vivo levels. CMTM 5 was among the differentially expressed genes (data from TCGA ). The expression of miR‐10b‐3p and CMTM 5 was detected by qRT ‐ PCR and Western blot ( WB ). TargetScan was used to acquire the binding sites. Dual‐luciferase reporter gene assay was used to verify the direct target relationship between miR‐10b‐3p and CMTM 5 . WB analysis proved that miR‐10b‐3p suppressed CMTM 5 expression. Furthermore, proliferation, invasion and migration of HCC cells were measured by MTT assay, colony formation assay, transwell assay and wound‐healing assay, respectively. Kaplan‐Meier plotter valued the overall survival of CMTM 5 . Finally, xenograft assay was also conducted to verify the effects of miR‐10b‐3p/ CMTM 5 axis in vivo. Up‐regulation of miR‐10b‐3p and down‐regulation of CMTM 5 were detected in HCC tissues and cell lines. CMTM 5 was verified as a target gene of miR‐10b‐3p. The overexpression of CMTM 5 contributed to the suppression of the proliferative, migratory and invasive abilities of HCC cells. Moreover, the up‐regulation of miR‐10b‐3p and down‐regulation of CMTM 5 were observed to be associated with worse overall survival. Lastly, we have confirmed the carcinogenesis‐related roles of miR‐10b‐3p and CMTM 5 in vivo. We concluded that the up‐regulation of miR‐10b‐3p promoted the progression of HCC cells via targeting CMTM 5 .