Open AccessLnc RNA NR 2F1‐ AS 1 regulates hepatocellular carcinoma oxaliplatin resistance by targeting ABCC 1 via miR‐363
Author(s) -
Huang Hai,
Chen Jie,
Ding ChengMing,
Jin Xin,
Jia ZeMing,
Peng Jian
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13605
Subject(s) - gene knockdown , oxaliplatin , rna , in vivo , microbiology and biotechnology , microrna , hepatocellular carcinoma , in vitro , cancer research , messenger rna , chemistry , competing endogenous rna , long non coding rna , biology , gene , cancer , biochemistry , colorectal cancer , genetics
Emerging evidence has validated the vital role of long non‐coding RNA (lnc RNA ) in the chemoresistance of cancer treatment. In the present study, we investigate the function of lnc RNA NR 2F1‐ AS 1 on oxaliplatin ( OXA ) resistance of hepatocellular carcinoma ( HCC ) and discover the underlying molecular mechanism. Results revealed that lnc RNA NR 2F1‐ AS 1 was up‐regulated in oxaliplatin‐resistant HCC tissue and cells using microarray analysis and RT ‐ PCR . Meanwhile, ABCC 1 protein was overexpressed in OXA ‐resistant HCC cells (Huh7/ OXA and HepG2/ OXA ). In vitro, NR 2F1‐ AS 1 knockdown reduced the invasion, migration, drug‐resistant gene ( MDR 1, MRP 5, LRP 1) and IC 50 value in Huh7/ OXA and HepG2/ OXA cells. In vivo, NR 2F1‐ AS 1 knockdown decreased the tumour weight of HCC cells. Bioinformatics tools and luciferase reporter assay confirmed miR‐363 targeted the 3′‐ UTR of NR 2F1‐ AS 1 and ABCC 1 mRNA , presenting that NR 2F1‐ AS 1 promoted ABCC 1 expression through endogenous sponging miR‐363. In summary, results conclude that NR 2F1‐ AS 1 regulates HCC OXA resistance through targeting miR‐363‐ ABCC 1 pathway, providing a vital theoretic mechanism and therapeutic target for HCC chemoresistance.