Open AccessLncRNA BDNF‐AS inhibits proliferation, migration, invasion and EMT in oesophageal cancer cells by targeting miR‐214
Author(s) -
Zhao Huaying,
Diao Changying,
Wang Xiaohui,
Xie Yilin,
Liu Yaqing,
Gao Xianzheng,
Han Jing,
Li Shenglei
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13558
Subject(s) - cell growth , cell migration , epithelial–mesenchymal transition , transfection , gentamicin protection assay , cell , microbiology and biotechnology , cancer research , chemistry , cancer cell , reporter gene , biology , cell culture , downregulation and upregulation , cancer , metastasis , gene expression , gene , biochemistry , genetics
This study was aimed at exploring the effect of lncRNA BDNF‐AS on cell proliferation, migration, invasion and epithelial‐to‐mesenchymal transition (EMT) of oesophageal cancer (EC) cells. The expression of BDNF‐AS and miR‐214 in tissue samples and cells was measured by qRT‐PCR. The targeted relationship between BDNF‐AS and miR‐214 was analysed by dual‐luciferase reporter assay. After cell transfection, the cell proliferation activity was assessed by MTS method, while the migrating and invading abilities were evaluated by transwell assay. LncRNA BDNF‐AS was remarkably down‐regulated, while miR‐214 was up‐regulated in EC tissues and cells in comparison with normal tissues and cells. Overexpression of BDNF‐AS significantly inhibited the abilities of cell proliferation, migration and invasion as well as the EMT processes of EC cells. The bioinformatics analysis and luciferase assay indicated that BDNF‐AS could be directly bound by miR‐214. Furthermore, overexpression of miR‐214 and BDNF‐AS exerted suppressive influence on EC cell multiplication, migration, invasion and EMT processes. LncRNA BDNF‐AS restrained cell proliferation, migration, invasion and EMT processes in EC cells by targeting miR‐214.