Open AccessETS 1 and SP 1 drive DHX 15 expression in acute lymphoblastic leukaemia
Author(s) -
Chen XiangLei,
Cai YuanHua,
Liu Qiao,
Pan LiLi,
Shi ShuiLing,
Liu XiaoLi,
Chen Yuan,
Li JingGang,
Wang Jing,
Li Yang,
Li XiaoFan,
Wang ShaoYuan
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13525
Subject(s) - gene knockdown , biology , gene , transcription (linguistics) , microbiology and biotechnology , promoter , transcription factor , gene expression , genetics , chromatin , linguistics , philosophy
DHX 15 plays a role in leukaemogenesis and leukaemia relapse. However, the mechanism underlying the transcriptional regulation of DHX 15 in ALL has not been elucidated. Our present study aimed to explore the functional promoter region of DHX 15 and to investigate the transcription factors controlling the transcription of this gene. A luciferase assay performed with several truncated constructs identified a 501‐bp region as the core promoter region of DHX 15 . Site‐directed mutagenesis, electrophoretic mobility shift and chromatin immunoprecipitation assays showed that ETS 1 and SP 1 occupied the DHX 15 promoter. Furthermore, knockdown of ETS 1 and SP 1 resulted in suppression of DHX 15 , whereas the overexpression of these genes led to up‐regulation of DHX 15 . Interestingly, in samples obtained from patients with ALL at diagnosis, both ETS 1 and SP 1 correlated positively with DHX 15 expression. Additionally, differences in methylation of the DHX 15 core promoter region were not observed between the patients and controls. In conclusion, we identified the core promoter region of DHX 15 and demonstrated that ETS 1 and SP 1 regulated DHX 15 expression in ALL .