Open AccessSPION ‐mediated miR‐141 promotes the differentiation of Hu AESC s into dopaminergic neuron‐like cells via suppressing lnc RNA ‐ HOTAIR
Author(s) -
Liu Te,
Zhang Hu,
Zheng Jiajia,
Lin Jiajia,
Huang Yongyi,
Chen Jiulin,
Yu Zhihua,
Guo Lihe,
Pan Weidong,
Xiong Ying,
Chen Chuan
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13512
Subject(s) - hotair , microrna , microbiology and biotechnology , western blot , biology , brain derived neurotrophic factor , neurotrophic factors , chemistry , rna , long non coding rna , gene , biochemistry , receptor
In this study, a bioinformatics analysis and luciferase reporter assay revealed that micro RNA ‐141 could silence the expression of lnc RNA ‐ HOTAIR by binding to specific sites on lnc RNA ‐ HOTAIR . We used superparamagnetic iron oxide nanoparticles ( SPION s) to mediate the high expression of micro RNA ‐141 ( SPION s@miR‐141) in human amniotic epithelial stem cells (Hu AESC s), which was followed by the induction of the differentiation of Hu AESC s into dopaminergic neuron‐like cells ( iDNLC s). qPCR , western blot, immunofluorescence staining and HPLC all suggested that SPION ‐mediated overexpression of miR‐141 could promote an increased expression of brain‐derived neurotrophic factor (BDNF), DAT and 5‐ TH in Hu AESC ‐derived iDNLC s. The RIP and Ch IP assay also showed that overexpression of miR‐141 could significantly inhibit the recruitment and binding of lnc RNA ‐ HOTAIR to EZH 2 on BDNF gene promoter. cDNA microarray analysis revealed that the expression levels of 190 genes were much higher in iDNLC s than in Hu AESC s. Finally, a protein interaction network analysis and identification showed that in the iDNLC group with SPION s@miR‐141, factors that interact with BDNF , such as FGF 8, SHH , NTRK 3 and CREB 1, all showed significantly higher expression levels compared with those in the SPION s@miR‐Mut. Therefore, this study confirmed that the highly efficient expression of micro RNA ‐141 mediated by SPION s could improve the efficiency of Hu AESC s differentiation into dopaminergic neuron‐like cells.