Open AccessHSP 27 associates with epithelial–mesenchymal transition, stemness and radioresistance of salivary adenoid cystic carcinoma
Author(s) -
Chen Wei,
Ren Xiaohua,
Wu Jiashun,
Gao Xiaolei,
Cen Xiao,
Wang Shasha,
Sheng Surui,
Chen Qianming,
Tang Yajie,
Liang Xinhua,
Tang Yaling
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13510
Subject(s) - radioresistance , adenoid cystic carcinoma , epithelial–mesenchymal transition , gene knockdown , biology , cancer research , cancer stem cell , stem cell , cancer , microbiology and biotechnology , metastasis , cell culture , carcinoma , genetics
Epithelial–mesenchymal transition ( EMT ) has been shown to associate with cancer stem cells and radioresistance. However, it is obscure whether EMT itself or specific EMT regulators play causal roles in these properties of salivary adenoid cystic carcinoma ( SACC ). Here, we exhibited that overexpression of HSP 27 drove the migration and invasion, induced EMT , as well as mediated TGF ‐β1‐induced EMT in SACC cells, accompanying the up‐regulation of Snail1 and Prrx1. Conversely, HSP 27 silencing reduced the migration and invasion and contributed to MET of SACC cells. HSP 27 indirectly down‐regulates the expression of E‐cadherin through activating Snail1 and Prrx1 expressions. Overexpression of Snail1 or Prrx1 restored the migration and invasion in HSP 27 knockdown cells. Enforced expression of HSP 27 enhanced colony formation, CD 133 + / CD 44 + population and radioresistance of SACC cell lines. In addition, HSP 27 expression was positively associated with radioresistance and poor prognosis of SACC patients as well as with the expression of Prrx1 or Snail1 in SACC tissues. The data confirm an important function for HSP 27 in SACC progression through regulating EMT and stemness, and they imply the possible association between EMT and radioresistance of SACC .