Open AccessRetracted: Effect of CLIC 1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
Author(s) -
He YueMing,
Zhang ZhongLin,
Liu QuanYan,
Xiao YuSha,
Wei Lei,
Xi Chen,
Nan Xiang
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13499
Subject(s) - apoptosis , cell cycle , cell growth , gene silencing , flow cytometry , gallbladder cancer , cell migration , cell , microbiology and biotechnology , messenger rna , biology , chemistry , cancer research , cancer , gene , biochemistry , genetics
This study aimed to explore the effects of CLIC 1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer ( GBC ). GBC and normal gallbladder tissues were extracted for the detection of mRNA and protein expressions of CLIC 1. GBC ‐ SD and NOZ cells in the logarithmic growth phase were selected to conduct the experiment. Three different si RNA recombined expression vectors were established using CLIC 1 as a target at different sites. Reverse transcription quantitative polymerase chain reaction ( RT ‐ qPCR ) and Western blotting were, respectively, used to detect the CLIC 1 mRNA and protein expressions. MTT assay was performed to detect the cell proliferation. Flow cytometry was applied to measure the cell apoptosis and cell cycle distribution. The variations of cell migration and invasion were evaluated using Transwell assay. GBC tissues showed higher CLIC 1 mRNA and protein expressions than normal gallbladder tissues. The CLIC 1 mRNA and protein expressions in the CLIC 1 si RNA group were significantly lower than those in the NC and blank groups. Compared with the NC and blank groups, the CLIC 1 si RNA group showed a significant decrease in cell proliferation but an obvious increase in apoptosis rate in GBC cells. Besides, in the CLIC 1 si RNA group, cell percentage in G0/G1 and G2/M phase was gradually increased but decreased in S phases. The migration and invasion abilities in GBC cells were significantly lower than those in the NC and blank groups. Our study demonstrates that CLIC 1 gene silencing could promote apoptosis and inhibit proliferation migration and invasion of GBC cells.