Open AccessStudy of platelet‐rich fibrin combined with rat periodontal ligament stem cells in periodontal tissue regeneration
Author(s) -
Duan Xuejing,
Lin Zhiyong,
Lin Xiujuan,
Wang Zhiqiang,
Wu Yihua,
Ji Mei,
Lu Wei,
Wang Xiaoyang,
Zhang Dongsheng
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13461
Subject(s) - bone sialoprotein , periodontal ligament stem cells , platelet rich fibrin , osteopontin , osteocalcin , periodontal fiber , chemistry , runx2 , alkaline phosphatase , regeneration (biology) , fibrin , tissue engineering , microbiology and biotechnology , dentistry , biomedical engineering , biology , medicine , immunology , biochemistry , enzyme
Abstract The objective of this study was to investigate the advantages and feasibility of periodontal tissue regeneration using platelet‐rich fibrin (PRF) combined with rat periodontal ligament stem cells (PDLSCs) for the first time. We first determined the effect of PRF on rat PDLSCs in vitro . We next conducted an in vivo study, in which a tissue engineering technique was performed to repair periodontal defects in five groups: a blank group, collagen group (implanted collagen membrane), collagen + cells group (implanted collagen membrane and rat PDLSCs), PRF group (implanted PRF membrane) and PRF + cells group (implanted PRF membrane and rat PDLSCs). PRF greatly enhanced cell proliferation, mRNA and protein expression levels of bone sialoprotein (BSP), osteocalcin (OC), and runt‐related transcription factor 2 (RUNX2) and activity of alkaline phosphatase (ALP) in vitro . Transplantation of PRF combined with rat PDLSCs resulted in higher expression of osteopontin (Opn), collagen I (COL1A) and RUNX2 at both 12 and 24 days after surgery. Micro‐computed tomography and histological analysis showed substantially more new bone formation in the PRF + cells group at 24 days after surgery. Based on these results, we discuss the role of PRF in the proliferation and differentiation of rat PDLSCs and suggest that PRF combined with rat PDLSCs provides a valuable tool for periodontal tissue engineering.