Open AccessBiological characteristics of human menstrual blood‐derived endometrial stem cells
Author(s) -
Liu Yanli,
Niu Rongcheng,
Yang Fen,
Yan Yan,
Liang Shengying,
Sun Yuliang,
Shen Ping,
Lin Juntang
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13437
Subject(s) - paracrine signalling , stem cell , endometrium , menstrual cycle , medicine , physiology , immune system , andrology , endocrinology , biology , immunology , microbiology and biotechnology , hormone , receptor
Successful isolation of human endometrial stem cells from menstrual blood, namely menstrual blood‐derived endometrial stem cells (Men SC s), has provided enticing alternative seed cells for stem cell‐based therapy. Men SC s are enriched in the self‐regenerative tissue, endometrium, which shed along the periodic menstrual blood and thus their acquisition involves no physical invasiveness. However, the impact of the storage duration of menstrual blood prior to stem cell isolation, the age of the donor, the number of passages on the self‐renewing of Men SC s, the paracrine production of biological factors in Men SC s and expression of adhesion molecules on Men SC s remain elusive. In this study, we confirmed that Men SC s reside in shedding endometrium, and documented that up to 3 days of storage at 4°C has little impact on Men SC s, while the age of the donor and the number of passages are negatively associated with proliferation capacity of Men SC s. Moreover, we found that Men SC s were actually immune‐privileged and projected no risk of tumour formation. Also, we documented a lung‐ and liver‐dominated, spleen‐ and kidney‐involved organic distribution profile of Men SC 3 days after intravenous transfer into mice. At last, we suggested that Men SC s may have potentially therapeutic effects on diseases through paracrine effect and immunomodulation.