Open AccessThe effects of conditioned media generated by polarized macrophages on the cellular behaviours of bone marrow mesenchymal stem cells
Author(s) -
He XiaoTao,
Li Xuan,
Yin Yuan,
Wu RuiXin,
Xu XinYue,
Chen FaMing
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13431
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , stem cell , bone marrow , fibronectin , extracellular matrix , phenotype , in vitro , transplantation , biology , stimulation , cellular differentiation , cell , chemistry , immunology , biochemistry , medicine , endocrinology , gene
Macrophages (Mφs) are involved in a variety of physiological and pathological events including wound healing and tissue regeneration, in which they play both positive and negative roles depending on their polarization state. In this study, we investigated the cellular behaviours of bone marrow mesenchymal stem cells ( BMMSC s) after incubation in different conditioned media ( CM s) generated by unpolarized Mφs (M0) or polarized Mφs (M1 and M2). Mφ polarization was induced by stimulation with various cytokines, and CM s were obtained from in vitro Mφ cultures termed CM 0, CM 1 and CM 2 based on each Mφ phenotype. We found that CM 1 supported the proliferation and adipogenic differentiation of BMMSC s, whereas CM 0 had a remarkable effect on cell osteogenic differentiation. To a certain degree, CM 2 also facilitated BMMSC osteogenesis; in particular, cells incubated with CM 2 exhibited an enhanced capacity to form robust stem cell sheets. Although incubation with CM 1 also increased production of extracellular matrix components, such as fibronectin, COL ‐1 and integrin β1during sheet induction, the sheets generated by CM 2‐incubated cells were thicker than those generated by CM 1‐incubated cells ( P < 0.001). Our data suggest that each Mφ phenotype has a unique effect on BMMSC s. Fine‐tuning Mφ polarization following transplantation may serve as an effective method to modulate the therapeutic potential of BMMSC s.