Open AccessPromotion of trophoblast invasion by lnc RNA MVIH through inducing Jun‐B
Author(s) -
Zou Yanfen,
Li Qian,
Xu Yetao,
Yu Xiang,
Zuo Qing,
Huang Shiyun,
Chu Yongli,
Jiang Ziyan,
Sun Lizhou
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13400
Subject(s) - angiogenesis , trophoblast , small interfering rna , gene silencing , biology , cell growth , microbiology and biotechnology , microrna , long non coding rna , cancer research , cell , transfection , rna , cell culture , placenta , gene , fetus , genetics , pregnancy
Preeclampsia ( PE ), a pregnancy‐specific disorder, is associated with impaired uterine spiral artery remodelling, which is related to the dysfunction of trophoblast cells. Lately, mounting evidence has indicated that aberrant expression of long non‐coding RNA s (lnc RNA s) is associated with various human diseases. The lnc RNA MVIH transcript has been shown to decrease the severity of several diseases. However, the biological function of MVIH , which is down‐regulated in placental tissues in PE , has not yet been clarified. Here, we report that MVIH may act as a vital factor in the pathogenesis of PE . In this study, functional analysis revealed that the silencing of MVIH expression via transfection with small interfering RNA (si RNA s) inhibited cell growth, migration, invasion, and angiogenesis in various trophoblast cell lines, and stimulation with MVIH could promote these functions. Mass spectrometry analysis revealed that MVIH could modulate Jun‐B protein expression, which has been reported to potentially regulate cell growth and angiogenesis. Further cotransfection assays were performed, revealing that MVIH and Jun‐B have a synergistic effect on the regulation of angiogenesis and cell proliferation. Taking these findings together, MVIH could be associated with PE and may be a candidate biomarker for its diagnosis and treatment.