Open AccessComparative analysis of serum proteome in congenital scoliosis patients with TBX 6 haploinsufficiency – a first report pointing to lipid metabolism
Author(s) -
Zhu Qiankun,
Wu Nan,
Liu Gang,
Zhou Yangzhong,
Liu Sen,
Chen Jun,
Liu Jiaqi,
Zuo Yuzhi,
Liu Zhenlei,
Chen Weisheng,
Chen Yixin,
Chen Jia,
Lin Mao,
Zhao Yanxue,
Yang Yang,
Wang Shensgru,
Yang Xu,
Ma Yufen,
Wang Jian,
Chen Xiaoli,
Zhang Jianguo,
Shen Jianxiong,
Wu Zhihong,
Qiu Guixing
Publication year - 2018
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13341
Subject(s) - haploinsufficiency , proteome , biology , microarray analysis techniques , gene ontology , bioinformatics , computational biology , genetics , gene , gene expression , phenotype
Congenital scoliosis ( CS ) is a three‐dimensional deformity of the spine affecting quality of life. We have demonstrated TBX 6 haploinsufficiency is the most important contributor to CS . However, the pathophysiology at the protein level remains unclear. Therefore, this study was to explore the differential proteome in serum of CS patients with TBX 6 haploinsufficiency. Sera from nine CS patients with TBX 6 haploinsufficiency and nine age‐ and gender‐matched healthy controls were collected and analysed by isobaric tagged relative and absolute quantification ( iTRAQ ) labelling coupled with mass spectrometry ( MS ). In total, 277 proteins were detected and 20 proteins were designated as differentially expressed proteins, which were submitted to subsequent bioinformatics analysis. Gene Ontology classification analysis showed the biological process was primarily related to ‘cellular process’, molecular function ‘structural molecule activity’ and cellular component ‘extracellular region’. IPA analysis revealed ‘ LXR / RXR activation’ was the top pathway, which is a crucial pathway in lipid metabolism. Hierarchical clustering analysis generated two clusters. In summary, this study is the first proteomic research to delineate the total and differential serum proteins in TBX 6 haploinsufficiency‐caused CS . The proteins discovered in this experiment may serve as potential biomarkers for CS , and lipid metabolism might play important roles in the pathogenesis of CS .