Open AccessLINC01101 and LINC00277 expression levels as novel factors in HPV‐induced cervical neoplasia
Author(s) -
Iancu Iulia Virginia,
Anton Gabriela,
Botezatu Anca,
Huica Irina,
Nastase Anca,
Socolov Demetra Gabriela,
Stanescu Anca Daniela,
Dima Simona Olimpia,
Bacalbasa Nicolae,
Plesa Adriana
Publication year - 2017
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13288
Subject(s) - biology , oncogene , transfection , hela , cervical cancer , microarray , gene expression , gene , cancer research , microarray analysis techniques , rna , small interfering rna , long non coding rna , cancer , microbiology and biotechnology , cell , genetics , cell cycle
Recently long non‐coding RNAs were identified as new factors involved in gene expression regulation. To gain insight into expression pattern of these factors related to E7 HPV18 oncogene, this study uses HeLa cell culture transfected with E7‐siRNA. Gene expression profile was investigated using microarray analysis. After analysing the microarray results, we identified 15,387 RNA species differentially expressed in E7‐siRNA‐transfected cells compared with controls (fold change >2). The expression profiles of lncRNA species highlighted 731 lncRNAs and 203 lincRNAs. We selected two lincRNAs (LINC01101 and LINC00277) and we evaluated the expression profile in HPV‐induced neoplasia. Both lincRNAs investigated display a significantly reduced pattern of expression in cervical lesions and cancer, associated with clinical parameters. A connection between HPV presence and lincRNAs was noted. hrHPV‐positive samples exhibit significantly reduced LINC01101 and LINC00277 expression level ( P < 0.05). These results provide new insights into involvement of lncRNA in HPV‐induced cervical cancer, enriching our understanding of their potential role in this pathology.