Open AccessMiR‐9 is involved in TGF ‐β1‐induced lung cancer cell invasion and adhesion by targeting SOX 7
Author(s) -
Han Lichun,
Wang Wei,
Ding Wei,
Zhang Lijian
Publication year - 2017
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13120
Subject(s) - cell adhesion , microrna , gene knockdown , matrigel , cancer research , gentamicin protection assay , cell growth , cell , cell culture , biology , cell adhesion molecule , cell migration , microbiology and biotechnology , chemistry , cancer , metastasis , angiogenesis , biochemistry , genetics , gene
MicroRNA (miR)‐9 plays different roles in different cancer types. Here, we investigated the role of miR‐9 in non‐small‐cell lung cancer ( NSCLC ) cell invasion and adhesion in vitro and explored whether miR‐9 was involved in transforming growth factor‐beta 1 ( TGF ‐β1)‐induced NSCLC cell invasion and adhesion by targeting SOX 7. The expression of miR‐9 and SOX 7 in human NSCLC tissues and cell lines was examined by reverse transcription‐quantitative polymerase chain reaction. Gain‐of‐function and loss‐of‐function experiments were performed on A549 and HCC 827 cells to investigate the effect of miR‐9 and SOX 7 on NSCLC cell invasion and adhesion in the presence or absence of TGF ‐β1. Transwell–Matrigel assay and cell adhesion assay were used to examine cell invasion and adhesion abilities. Luciferase reporter assay was performed to determine whether SOX 7 was a direct target of miR‐9. We found miR‐9 was up‐regulated and SOX 7 was down‐regulated in human NSCLC tissues and cell lines. Moreover, SOX 7 expression was negatively correlated with miR‐9 expression. miR‐9 knockdown or SOX 7 overexpression could suppress TGF ‐β1‐induced NSCLC cell invasion and adhesion. miR‐9 directly targets the 3′ untranslated region of SOX 7, and SOX 7 protein expression was down‐regulated by miR‐9. TGF ‐β1 induced miR‐9 expression in NSCLC cells. miR‐9 up‐regulation led to enhanced NSCLC cell invasion and adhesion; however, these effects could be attenuated by SOX 7 overexpression. We concluded that miR‐9 expression was negatively correlated with SOX 7 expression in human NSCLC . miR‐9 was up‐regulated by TGF ‐β1 and contributed to TGF ‐β1‐induced NSCLC cell invasion and adhesion by directly targeting SOX 7.