Open AccessNK 1.1 − CD 4 + NKG 2D + T cells suppress DSS ‐induced colitis in mice through production of TGF ‐β
Author(s) -
Qian Xingxing,
Hu Chunxia,
Han Sen,
Lin Zhijie,
Xiao Weiming,
Ding Yanbing,
Zhang Yu,
Qian Li,
Jia Xiaoqing,
Zhu Guoqiang,
Gong Weijuan
Publication year - 2017
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13072
Subject(s) - nkg2d , immune system , interleukin 12 , interleukin 21 , cytotoxic t cell , microbiology and biotechnology , immunology , chemistry , t cell , biology , biochemistry , in vitro
CD 4 + NKG 2D + T cells are associated with tumour, infection and autoimmune diseases. Some CD 4 + NKG 2D + T cells secrete IFN ‐γ and TNF ‐α to promote inflammation, but others produce TGF ‐β and FasL to facilitate tumour evasion. Here, murine CD 4 + NKG 2D + T cells were further classified into NK 1.1 − CD 4 + NKG 2D + and NK 1.1 + CD 4 + NKG 2D + subpopulations. The frequency of NK 1.1 − CD 4 + NKG 2D + cells decreased in inflamed colons, whereas more NK 1.1 + CD 4 + NKG 2D + cells infiltrated into colons of mice with DSS ‐induced colitis. NK 1.1 − CD 4 + NKG 2D + cells expressed TGF ‐β and FasL without secreting IFN ‐γ, IL ‐21 and IL ‐17 and displayed no cytotoxicity. The adoptive transfer of NK 1.1 − CD 4 + NKG 2D + cells suppressed DSS ‐induced colitis largely dependent on TGF ‐β. NK 1.1 − CD 4 + NKG 2D + cells did not expressed Foxp3, CD 223 ( LAG ‐3) and GITR . The subpopulation was distinct from NK 1.1 + CD 4 + NKG 2D + cells in terms of surface markers and RNA transcription. NK 1.1 − CD 4 + NKG 2D + cells also differed from Th2 or Th17 cells because the former did not express GATA ‐3 and ROR ‐γt. Thus, NK 1.1 − CD 4 + NKG 2D + cells exhibited immune regulatory functions, and this T cell subset could be developed to suppress inflammation in clinics.