Open AccessTelocytes: a potential defender in the spleen of Npc1 mutant mice
Author(s) -
Zhang Bichao,
Yang Ciqing,
Qiao Liang,
Li Qiuling,
Wang Congrui,
Yan Xin,
Lin Juntang
Publication year - 2017
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13024
Subject(s) - mutant , spleen , npc1 , biology , microbiology and biotechnology , immunology , genetics , gene , endosome , intracellular
Niemann–Pick disease, type C1 (Npc1), is an atypical lysosomal storage disorder caused by autosomal recessive inheritance of mutations in Npc1 gene. In the Npc1 mutant mice (Npc1 −/− ), the initial manifestation is enlarged spleen, concomitant with free cholesterol accumulation. Telocytes (TCs), a novel type of interstitial cell, exist in a variety of tissues including spleen, presumably thought to be involved in many biological processes such as nursing stem cells and recruiting inflammatory cells. In this study, we found that the spleen is significantly enlarged in Npc1 −/− mice, and the results from transmission electron microscopy examination and immunostaining using three different TCs markers, c‐Kit, CD34 and Vimentin revealed significantly increased splenic TCs in Npc1 −/− mice. Furthermore, hematopoietic stem cells and macrophages were also elevated in Npc1 −/− spleen. Taken together, our data indicate that splenic TCs might alleviate the progress of splenic malfunction via recruiting hematopoietic stem cells and macrophages.