Open AccessNCAPG 2 promotes tumour proliferation by regulating G2/M phase and associates with poor prognosis in lung adenocarcinoma
Author(s) -
Zhan Ping,
Xi Guangmin,
Zhang Bin,
Wu Ying,
Liu Hongbing,
Liu Yafang,
Xu Wujian,
Zhu Qingqing,
Cai Feng,
Zhou Zejun,
Miu Yingying,
Wang Xiaoxia,
Jin Jiajia,
Li Qian,
Lv Tangfeng,
Song Yong
Publication year - 2017
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.13010
Subject(s) - biology , cell cycle , cyclin dependent kinase 1 , cell growth , mitosis , gene silencing , cancer research , gene knockdown , adenocarcinoma , cell cycle checkpoint , oncogene , cyclin d1 , cell , cancer , microbiology and biotechnology , genetics , gene
NCAPG 2 is a component of the condensin II complex and contributes to chromosome segregation via microtubule–kinetochore attachment during mitosis. It is well known that NCAPG 2 plays a critical role in cell mitosis; however, the role of altered NCAPG 2 expression and its transcriptional regulatory function in cancer development remains mostly unknown. Here, for the first time we reported that NCAPG 2 was evidently increased in non‐small cell lung cancer tissues compared to adjacent normal lung tissues. Clinicopathological data analysis showed that NCAPG 2 overexpression was significantly correlated with lymph node metastasis and pathologic‐Tumour Nodes Metastasen stages, and was an independent prognostic factor in lung adenocarcinoma patients. Moreover, si RNA ‐mediated knockdown of NCAPG 2 could inhibit tumour cell growth of lung adenocarcinoma cells (A549 and H1299) in vitro and could significantly lead to cell cycle arrest in the G2 phase. Furthermore, we found that NCAPG 2 silencing significantly decreased the expression levels of G2/M phase cell cycle‐related protein expressions (Cyclin B1, Cdc2) and increased the expression levels of p27 and p21 through Western blot analysis. Taken together, we demonstrated that increased NCAPG 2 expression could regulate cell proliferation and identified as a poor prognostic biomarker in lung adenocarcinoma.