Open AccessGenetic variants of nuclear factor erythroid‐derived 2‐like 2 associated with the complications in Han descents with type 2 diabetes mellitus of Northeast China
Author(s) -
Xu Xiaohong,
Sun Jing,
Chang Xiaomin,
Wang Ji,
Luo Manyu,
Wintergerst Kupper A.,
Miao Lining,
Cai Lu
Publication year - 2016
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12900
Subject(s) - genotype , snp , medicine , single nucleotide polymorphism , genotyping , diabetes mellitus , haplotype , nephropathy , allele , gastroenterology , biology , bioinformatics , endocrinology , genetics , gene
The transcription factor nuclear factor erythroid 2‐like 2 ( NFE 2L2) is essential for preventing type 2 diabetes mellitus (T2 DM )‐induced complications in animal models. This case and control study assessed genetic variants of NFE 2L2 for associations with T2 DM and its complications in Han Chinese volunteers. T2 DM patients with ( n = 214) or without ( n = 236) complications, or healthy controls ( n = 359), were genotyped for six NFE 2L2 single nucleotide polymorphisms ( SNP s: rs2364723, rs13001694, rs10497511, rs1806649, rs1962142 and rs6726395) with TaqMan Pre‐Designed SNP Genotyping and Sequence System. Serum levels of heme oxygenase‐1 ( HMOX 1) were determined through enzyme‐linked immunosorbent assay. Informative data were obtained for 341 cases and 266 controls. Between T2 DM patients and controls, the genotypic and allelic frequencies and haplotypes of the SNP s were similar. However, there was a significant difference in genotypic and allelic frequencies of rs2364723, rs10497511, rs1962142 and rs6726395 between T2 DM patients with and without complications, including peripheral neuropathy, nephropathy, retinopathy, foot ulcers and microangiopathy. Furthermore, HMOX 1 levels were significantly higher in T2 DM patients with complications than in controls. Multiple logistic regression analysis, however, showed that only rs2364723 significantly reduced levels of serum HMOX 1 in T2 DM patients for the GG genotype carriers compared with participants with CG + CC genotype. The data suggest that although NFE 2L2 rs2364723, rs10497511, rs1962142 and rs6726395 were not associated with T2 DM risk, they were significantly associated with complications of T2 DM . In addition, only for rs2364723 higher serum HMOX 1 levels were found in the T2 DM patients with CG + CC than those with GG genotype.