Open AccessHelium‐based cold atmospheric plasma‐induced reactive oxygen species‐mediated apoptotic pathway attenuated by platinum nanoparticles
Author(s) -
Jawaid Paras,
Rehman Mati Ur,
Zhao Qing Li,
Takeda Keigo,
Ishikawa Kenji,
Hori Masaru,
Shimizu Tadamichi,
Kondo Takashi
Publication year - 2016
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12880
Subject(s) - reactive oxygen species , apoptosis , u937 cell , chemistry , biophysics , catalase , superoxide , oxidative stress , microbiology and biotechnology , biochemistry , biology , enzyme
Plasma is generated by ionizing gas molecules. Helium (He)‐based cold atmospheric plasma ( CAP ) was generated using a high‐voltage power supply with low‐frequency excitation (60 Hz at 7 kV) and He flow at 2 l/min. Platinum nanoparticles (Pt‐ NP s) are potent antioxidants due to their unique ability to scavenge superoxides and peroxides. These features make them useful for the protection against oxidative stress‐associated pathologies. Here, the effects of Pt‐ NP s on He‐ CAP ‐induced apoptosis and the underlying mechanism were examined in human lymphoma U937 cells. Apoptosis was measured after cells were exposed to He‐ CAP in the presence or absence of Pt‐ NP s. The effects of combined treatment were determined by observing the changes in intracellular reactive oxygen species ( ROS ) and both mitochondrial and Fas dependent pathway. The results indicate that Pt‐ NP s substantially scavenge He‐ CAP ‐induced superoxides and peroxides and inhibit all the pathways involved in apoptosis execution. This might be because of the SOD /catalase mimetic effects of Pt‐ NP s. These results showed that the Pt‐ NP s can induce He‐ CAP desensitization in human lymphoma U937 cells.