Open AccessMinocycline attenuates post‐operative cognitive impairment in aged mice by inhibiting microglia activation
Author(s) -
Wang HuiLin,
Liu Hua,
Xue ZhangGang,
Liao QingWu,
Fang Hao
Publication year - 2016
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12854
Subject(s) - microglia , neuroprotection , hippocampal formation , isoflurane , western blot , morris water navigation task , cytokine , minocycline , medicine , inflammation , pharmacology , hippocampus , tumor necrosis factor alpha , barnes maze , neuroscience , immunology , anesthesia , psychology , biology , biochemistry , spatial learning , gene , antibiotics
Although it is known that isoflurane exposure or surgery leads to post‐operative cognitive dysfunction in aged rodents, there are few clinical interventions and treatments available to prevent this disorder. Minocycline ( MINO ) produces neuroprotection from several neurodegenerative diseases and various experimental animal models. Therefore, we set out to investigate the effects of MINO pre‐treatment on isoflurane or surgery induced cognitive impairment in aged mice by assessing the hippocampal‐dependent spatial memory performance using the Morris water maze task. Hippocampal tissues were isolated from mice and evaluated by Western blot analysis, immunofluorescence procedures and protein array system. Our results elucidate that MINO down‐regulated the isoflurane‐induced and surgery‐induced enhancement in the protein levels of pro‐inflammatory cytokine tumour necrosis factor alpha, interleukin ( IL )‐1β, interferon‐γ and microglia marker Iba‐1, and up‐regulated protein levels of the anti‐inflammatory cytokine IL ‐4 and IL ‐10. These findings suggest that pre‐treatment with MINO attenuated isoflurane or surgery induced cognitive impairment by inhibiting the overactivation of microglia in aged mice.