Open AccessInternalization of nanopolymeric tracers does not alter characteristics of placental cells
Author(s) -
Bigini Paolo,
Zanier Elisa R.,
Saragozza Silvia,
Maciotta Simona,
Romele Pietro,
Bonassi Signoroni Patrizia,
Silini Antonietta,
Pischiutta Francesca,
Sammali Eliana,
Balducci Claudia,
Violatto Martina B.,
Talamini Laura,
Garry David,
Moscatelli Davide,
Ferrari Raffaele,
Salmona Mario,
De Simoni Maria Grazia,
Maggi Federico,
Simoni Giuseppe,
Grati Francesca Romana,
Parolini Ornella
Publication year - 2016
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12820
Subject(s) - mesenchymal stem cell , in vivo , cell therapy , in vitro , internalization , placenta , immune system , chemistry , cell , microbiology and biotechnology , stromal cell , stem cell , pharmacology , cancer research , immunology , medicine , fetus , biology , pregnancy , biochemistry , genetics
In the cell therapy scenario, efficient tracing of transplanted cells is essential for investigating cell migration and interactions with host tissues. This is fundamental to provide mechanistic insights which altogether allow for the understanding of the translational potential of placental cell therapy in the clinical setting. Mesenchymal stem/stromal cells ( MSC ) from human placenta are increasingly being investigated for their potential in treating patients with a variety of diseases. In this study, we investigated the feasibility of using poly (methyl methacrylate) nanoparticles ( PMMA ‐ NP s) to trace placental MSC , namely those from the amniotic membrane ( hAMSC ) and early chorionic villi ( hCV ‐ MSC ). We report that PMMP ‐ NP s are efficiently internalized and retained in both populations, and do not alter cell morphofunctional parameters. We observed that PMMP ‐ NP incorporation does not alter in vitro immune modulatory capability of placental MSC , a characteristic central to their reparative/therapeutic effects in vitro . We also show that in vitro , PMMP ‐ NP uptake is not affected by hypoxia. Interestingly, after in vivo brain ischaemia and reperfusion injury achieved by transient middle cerebral artery occlusion ( tMCA o) in mice, iv hAMSC treatment resulted in significant improvement in cognitive function compared to PBS ‐treated tMCA o mice. Our study provides evidence that tracing placental MSC with PMMP ‐ NP s does not alter their in vitro and in vivo functions. These observations are grounds for the use of PMMP ‐ NP s as tools to investigate the therapeutic mechanisms of hAMSC and hCV ‐ MSC in preclinical models of inflammatory‐driven diseases.