Open AccessProtein profile of basal prostate epithelial progenitor cells—stage‐specific embryonal antigen 4 expressing cells have enhanced regenerative potential in vivo
Author(s) -
Höfner Thomas,
Klein Corinna,
Eisen Christian,
RigoWatermeier Teresa,
Haferkamp Axel,
Sprick Martin R.
Publication year - 2016
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12785
Subject(s) - progenitor cell , stem cell , biology , progenitor , transplantation , cancer research , immunology , microbiology and biotechnology , medicine
The long‐term propagation of basal prostate progenitor cells ex vivo has been very difficult in the past. The development of novel methods to expand prostate progenitor cells in vitro allows determining their cell surface phenotype in greater detail. Mouse (Lin − Sca‐1 + CD 49f + Trop2 high ‐phenotype) and human (Lin − CD 49f + TROP 2 high ) basal prostate progenitor cells were expanded in vitro . Human and mouse cells were screened using 242 anti‐human or 176 antimouse monoclonal antibodies recognizing the cell surface protein profile. Quantitative expression was evaluated at the single‐cell level using flow cytometry. Differentially expressed cell surface proteins were evaluated in conjunction with the known CD 49f + / TROP 2 high phenotype of basal prostate progenitor cells and characterized by in vivo sandwich‐transplantation experiments using nude mice. The phenotype of basal prostate progenitor cells was determined as CD 9 + / CD 24 + / CD 29 + / CD 44 + / CD 47 + / CD 49f + / CD 104 + / CD 147 + / CD 326 + /Trop2 high of mouse as well as human origin. Our analysis revealed several proteins, such as CD 13, Syndecan‐1 and stage‐specific embryonal antigens ( SSEA s), as being differentially expressed on murine and human CD 49f + TROP 2 + basal prostate progenitor cells. Transplantation experiments suggest that CD 49f + TROP 2 high SSEA ‐4 high human prostate basal progenitor cells to be more potent to regenerate prostate tubules in vivo as compared with CD 49f + TROP 2 high or CD 49f + TROP 2 high SSEA ‐4 low cells. Determination of the cell surface protein profile of functionally defined murine and human basal prostate progenitor cells reveals differentially expressed proteins that may change the potency and regenerative function of epithelial progenitor cells within the prostate. SSEA ‐4 is a candidate cell surface marker that putatively enables a more accurate identification of the basal PESC lineage.