Open AccessPlasma from pre‐eclamptic patients induces the expression of the anti‐angiogenic miR‐195‐5p in endothelial cells
Author(s) -
Sandrim Valeria Cristina,
Dias Mayara Caldeira,
Bovolato Ana Lívia de Carvalho,
TanusSantos Jose E,
Deffune Elenice,
Cavalli Ricardo C.
Publication year - 2016
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12767
Subject(s) - preeclampsia , incubation , andrology , plasma levels , vegf receptors , gene expression , vascular endothelial growth factor a , endocrinology , medicine , chemistry , biology , vascular endothelial growth factor , pregnancy , gene , biochemistry , genetics
Abstract We examined the effect of plasma incubation from preeclampsia pregnant on the antiangiogenic miR‐195‐5p expression. Higher miR‐195‐5p expression was found in cultures incubated with preeclampsia plasma compared to those incubated with healthy pregnant plasma. Next, as VEGF is a target of miR‐195‐5p we have quantified its expression by real‐time qPCR and ELISA. We found reduced VEGF levels in culture incubated with preeclampsia plasma. Therefore, we have concluded that the higher expression of miR‐195‐5p in endothelial cell cultures incubated with preeclampsia plasma may contribute to decreased expression of VEGFA (gene and protein) and increased antiangiogenic status in preeclampsia. Therefore, this miR may be an important target in preeclampsia.