z-logo
open-access-imgOpen Access
Transmembrane domain is crucial to the subcellular localization and function of Myc target 1
Author(s) -
Wu Shuai,
Gui Jinghua,
Yin Xiaofei,
Pan Qiang,
Liu Xinyuan,
Chu Liang
Publication year - 2016
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12747
Subject(s) - transmembrane protein , subcellular localization , biology , microbiology and biotechnology , nuclear localization sequence , cell growth , transmembrane domain , viability assay , cytoplasm , cell , apoptosis , mutation , phenotype , gene , genetics , receptor
Deregulation of c‐ MYC occurs in a variety of human cancers. Overexpression of c‐ MYC promotes cell growth, proliferation, apoptosis, transformation and genomic instability. MYC target 1 ( MYCT 1) is a direct target gene of c‐ MYC , and its murine homologue MT ‐ MC 1 recapitulated multiple c‐Myc‐related phenotypes. However, the molecular mechanism of MYCT 1 remains unclear. Here, we identified the transmembrane ( TM ) domain of MYCT 1, not the nuclear localization sequence, is indispensable to the vesicle‐associated localization of MYCT 1 protein in the cytoplasmic membrane vesicle. Overexpression of MYCT 1, not MYCT 1 (Δ TM ), decreased cell viability under serum deprivation and increased tumour cell migration ability. We further identified CKAP 4 interacted with MYCT 1 and contributed to the function of MYCT 1. In addition, we found that a mutation, A88D, which is observed in patient sample, changed the localization, and abolished the effect on cell viability and cell migration, suggesting that the TM domain is critical to MYCT 1.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here