Open AccessChanges in different parameters, lymphocyte proliferation and hematopoietic progenitor colony formation in EAE mice treated with myelin oligodendrocyte glycoprotein
Author(s) -
Doronin Vasilii B.,
Parkhomenko Taisiya A.,
Korablev Alexey,
Toporkova Ludmila B.,
Lopatnikova Julia A.,
Alshevskaja Alina A.,
Sennikov Sergei V.,
Buneva Valenti.,
Budde Thomas,
Meuth Sven G.,
Orlovskaya Irina A.,
Popova Nelly A.,
Nevinsky Georgy A.
Publication year - 2016
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12704
Subject(s) - myelin oligodendrocyte glycoprotein , haematopoiesis , glycoprotein , immunology , progenitor cell , myelin , myelin basic protein , biology , progenitor , lymphocyte , oligodendrocyte , virology , microbiology and biotechnology , stem cell , multiple sclerosis , experimental autoimmune encephalomyelitis , central nervous system , neuroscience
Abstract Myelin oligodendrocyte glycoprotein ( MOG ) is an antigen of the myelin sheath, which may trigger immune cell responses and the production of auto‐antibodies in multiple sclerosis ( MS ). In this study, we used MOG 35‐55 ‐induced experimental autoimmune encephalomyelitis ( EAE ), a model of human MS , to assess the production of catalytically active immunoglobulin G (IgG) antibodies or abzymes which have been shown to be present in sera of patients with several autoimmune diseases. Here, we show that IgGs from the sera of control C57 BL /6 mice are catalytically inactive. During development of EAE , a specific reorganization of the immune system of mice occurred leading to a condition which was associated with the generation of catalytically active IgGs hydrolysing DNA , myelin basic protein ( MBP ) and MOG which was associated with increased proteinuria, changes in differentiation of mice bone marrow hematopoietic stem cells ( HSC s) and an increase in proliferation of lymphocytes in bone marrow, spleen and thymus as well as a significant suppression of cell apoptosis in these organs. The strongest alterations were found in the early disease phase (18–24 days after immunization) and were less pronounced in later EAE stages (40 days after EAE induction). We conclude that a significant increase in DN ase and proteolytic activities of antibodies may be considered the earliest statistically significant marker of MOG ‐induced EAE in mice. The possible differences in immune system reorganizations during preclinical phases of the disease, acute and late EAE , leading to production of different auto‐antibodies and abzymes as well other changes are discussed.