Open AccessIn vitro expression of cytokeratin 18, 19 and tube formation of adipose‐derived stem cells induced by the breast epithelial cell line HBL ‐100
Author(s) -
Yang Jie,
Xiong Lingyun,
Wang Rongrong,
Yuan Quan,
Xia Yun,
Sun Jiaming,
Horch Raymund E.
Publication year - 2015
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12673
Subject(s) - cytokeratin , adipose tissue , stem cell , biology , transplantation , pathology , cell culture , cell , epithelium , microbiology and biotechnology , immunology , medicine , immunohistochemistry , endocrinology , genetics
Abstract Fat transplantation is increasingly used in breast augmentation; and recently, the issue of safety concerns from a cellular and molecular point of view has been raised. In this study, attentions were paid to the interaction between adipose‐derived stem cells ( ADSC ) and mammary epithelial cells: human breast cancer cell line ‐ 100 (HBL ‐ 100) cells were used to simulate the normal microenvironment in breast tissue, ADSC s were harvest from human and co‐cultured with HBL ‐100 cells. It was found that ADSC s formed tube‐like structures in the co‐culture with HBL ‐100 cells in contrast to the normal morphology of ADSC s in the control group. In addition, the immunofluorescence imaging showed that cytokeratin 18 and 19 ( CK 18 and 19) were significantly expressed in ADSC s after the co‐culture with HBL ‐100 cells. The ultrastructure of those ADSC s also showed epithelial changes. In conclusion, ADSC s are not biological stable when co‐cultured with HBL ‐100 cells. They differentiate into epithelial‐like cells with the expression of epithelial surface marks ( CK 18, 19) and form tube‐like structures. This may offer an important evidence for the further study of clinical application of transplanting ADSC s rich adipose tissue into the breast in the future.