Open AccessThe impact of inflammatory cells in malignant ascites on small intestinal ICCs' morphology and function
Author(s) -
Li Jing,
Kong Dan,
He Yan,
Wang Xiuli,
Gao Lei,
Li Jiade,
Yan Meisi,
Liu Duanyang,
Wang Yufu,
Zhang Lei,
Jin Xiaoming
Publication year - 2015
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12575
Subject(s) - ascites , interstitial cell of cajal , motility , gastrointestinal tract , pathology , abdominal cavity , medicine , inflammation , biology , immunohistochemistry , anatomy , genetics
Malignant ascites is one of the common complication at the late stage of abdominal cancers, which may deteriorate the environment of abdominal cavity and lead to potential damage of functional cells. Interstitial cells of Cajal ( ICC s) are mesoderm‐derived mesenchymal cells that function normal gastrointestinal motility. The pathological changes of ICC s or the reduced number may lead to the motility disorders of gastrointestinal tract. In this study, through analysis of malignant ascites which were obtained from cancer patients, we found that inflammatory cells, including tumour‐infiltrating lymphocytes, accounted for 17.26 ± 1.31% and tumour‐associated macrophages, occupied 19.06 ± 2.27% of total cells in the ascites, suggesting these inflammatory cells, in addition to tumour cells, may exert important influence on the tumour environment of abdominal cavity. We further demonstrated that the number of mice ICC s were significant decreased, as well as morphological and functional damage when ICC s were in the simulated tumour microenvironment in vitro . Additionally, we illustrated intestinal myoelectrical activity reduced and irregular with morphological changes of ICC s using the mice model of malignant ascites. In conclusion, our data suggested that inflammatory cells in malignant ascites may damage ICC s of the small intestine and lead to intestinal motility disorders.