Open AccessAcacetin inhibits expression of matrix metalloproteinases via a MAPK ‐dependent mechanism in fibroblast‐like synoviocytes
Author(s) -
Chen WeiPing,
Yang ZhiGao,
Hu PengFei,
Bao JiaPeng,
Wu LiDong
Publication year - 2015
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12564
Subject(s) - matrix metalloproteinase , microbiology and biotechnology , mechanism (biology) , chemistry , mapk/erk pathway , fibroblast , signal transduction , biology , biochemistry , physics , quantum mechanics , in vitro
It is well known that rheumatoid arthritis ( RA ) is an autoimmune joint disease in which fibroblast‐like synoviocytes ( FLS s) play a pivotal role. In this study, we investigated the anti‐arthritic properties of acacetin in FLS s. The expression of matrix metalloproteinase ( MMP )‐1, MMP ‐3 and MMP ‐13 were investigated by quantitative RT ‐ PCR and western blot at gene and protein levels. At the same time, the phosphorylation of mitogen‐activated protein kinases ( MAPK ) was investigated. The DNA ‐binding activity of NF ‐κB was investigated by electrophoretic mobility shift assay. We found that acacetin inhibits p38 and JNK phosphorylation and reduces MMP ‐1, MMP ‐3 and MMP ‐13 expression in interleukin‐1β‐induced FLS s. Our results suggest that acacetin has antiarthritic effects in FLS s. Thus, acacetin should be further studied for the treatment of arthritis.