Open AccessDiallyl trisulfide inhibits migration, invasion and angiogenesis of human colon cancer HT ‐29 cells and umbilical vein endothelial cells, and suppresses murine xenograft tumour growth
Author(s) -
Lai KuangChi,
Hsu ShuChun,
Yang JaiSing,
Yu ChienChih,
Lein JinCherng,
Chung JingGung
Publication year - 2015
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12486
Subject(s) - angiogenesis , diallyl trisulfide , umbilical vein , cancer research , chorioallantoic membrane , human umbilical vein endothelial cell , chemistry , in vivo , angiogenesis inhibitor , cancer cell , biology , microbiology and biotechnology , cancer , medicine , in vitro , biochemistry , apoptosis
Angiogenesis inhibitors are beneficial for the prevention and treatment of angiogenesis‐dependent diseases including cancer. We examined the cytotoxic, anti‐metastatic, anti‐cancer and anti‐angiogenic effects of diallyl trisulfide ( DATS ). In HT 29 cells, DATS inhibited migration and invasion through the inhibition of focal adhesion kinase ( FAK ), extracellular signal‐regulated kinase, c‐Jun N‐terminal kinase and p38 which was associated with inhibition of matrix metalloproteinases‐2, ‐7 and ‐9 and VEGF . In human umbilical vein endothelial cells ( HUVEC ), DATS inhibited the migration and angiogenesis through FAK , Src and Ras. DATS also inhibited the secretion of VEGF . The capillary‐like tube structure formation and migration by HUVEC was inhibited by DATS . The chicken egg chorioallantoic membrane ( CAM ) assay indicated that DATS treatment inhibited ex‐vivo angiogenesis. We investigated the anti‐tumour effects of DATS against human colon cancer xenografts in BALB /c nu/nu mice and its anti‐angiogenic activity in vivo . In this in‐vivo study, DATS also inhibited the tumour growth, tumour weight and angiogenesis (decreased the levels of haemoglobin) in HT 29 cells. In conclusion, the present results suggest that the inhibition of angiogenesis may be an important mechanism in colon cancer chemotherapy by DATS .