Open AccessDifferent types of cultured human adult Cardiac Progenitor Cells have a high degree of transcriptome similarity
Author(s) -
Gaetani Roberto,
Feyen Dries A. M.,
Doevendans Pieter A.,
Gremmels Hendrik,
Forte Elvira,
Fledderus Joost O.,
Ramjankhan Faiz Z.,
Messina Elisa,
Sussman Mark A.,
Giacomello Alessandro,
Sluijter Joost P. G.
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12458
Subject(s) - transcriptome , similarity (geometry) , progenitor cell , biology , degree (music) , computational biology , progenitor , microbiology and biotechnology , stem cell , genetics , gene , gene expression , computer science , artificial intelligence , physics , acoustics , image (mathematics)
Taken together, our data suggest that human CPCs can be iso-\udlated from patient heart biopsies using different markers, such as\udc-kit or Sca-1- like, and alternative methodologies,\udvia\uddirect cell iso-\udlation or\udvia\udexplant culture, such as CSps and CDCs. For the first\udtime, however, we showed that upon culture expansion, these cell\udpopulations have a very similar gene expression profile, even more\udpronounced when cultured in comparable culture conditions and even\udtranscended by donor differences. Our findings are of fundamental importance to create a con-\udsensus among different scientists in the field of myocardial regenera-\udtion, which should help align future clinical approaches to improve\udthe reported beneficial effects of cell therapy for heart disease by\udusing cardiac derived progenitor cell populations