Open AccessMicro RNA ‐30d and micro RNA ‐181a regulate HOXA 11 expression in the uterosacral ligaments and are overexpressed in pelvic organ prolapse
Author(s) -
Jeon Myung Jae,
Kim Eun Jae,
Lee Maria,
Kim Hoguen,
Choi Jong Rak,
Chae Hee Dong,
Moon Yeo Jung,
Kim Sei Kwang,
Bai Sang Wook
Publication year - 2015
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12448
Subject(s) - luciferase , gene knockdown , messenger rna , rna , microbiology and biotechnology , chemistry , transfection , biology , gene , biochemistry
The balanced turnover of collagen is necessary to maintain the mechanical strength of pelvic supportive connective tissues. Homeobox ( HOX ) A11 is a key transcriptional factor that controls collagen metabolism and homoeostasis in the uterosacral ligaments ( USL s), and the deficient HOXA 11 signalling may contribute to alterations in the biochemical strength of the USL s, leading to pelvic organ prolapse ( POP ). However, it is unknown how HOXA 11 transcripts are regulated in the USL s. In this study, we found that micro RNA (mi RNA )‐30d and 181a were overexpressed in women with POP , and their expression was inversely correlated with HOXA 11 mRNA levels. The overexpression of miR‐30d or 181a suppressed HOXA 11 mRNA and protein levels in 293T cells, whereas the knockdown of these mi RNA s enhanced HOXA 11 levels and collagen production. Cotransfection of a luciferase reporter plasmid containing the 3′‐untranslated region of HOXA 11 with miR‐30d or 181a mimic resulted in decreased relative luciferase activity. Conversely, cotransfection with anti‐miR‐30d or 181a increased luciferase activity. Taken together, these results indicate that both miR‐30d and 181a are important posttranscriptional regulators of HOXA 11 in the USL s and could be a potential therapeutic target for POP .