Open AccessFabp4‐Cre ER lineage tracing revealstwo distinctive coronary vascular populations
Author(s) -
He Lingjuan,
Tian Xueying,
Zhang Hui,
Wythe Joshua D.,
Zhou Bin
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12415
Subject(s) - lineage (genetic) , biology , population , heart development , pathological , pathology , embryonic stem cell , medicine , genetics , gene , environmental health
Over the last two decades, genetic lineage tracing has allowed for the elucidation of the cellular origins and fates during both embryogenesis and in pathological settings in adults. Recent lineage tracing studies using Apln‐Cre ER tool indicated that a large number of post‐natal coronary vessels do not form from pre‐existing vessels. Instead, they form de novo after birth, which represents a coronary vascular population ( CVP ) distinct from the pre‐existing one. Herein, we present new coronary vasculature lineage tracing results using a novel tool, Fabp4‐Cre ER . Our results confirm the distinct existence of two unique CVP s. The 1 st CVP , which is labelled by Fabp4‐Cre ER , arises through angiogenic sprouting of pre‐existing vessels established during early embryogenesis. The 2 nd CVP is not labelled by Fabp4, suggesting that these vessels form de novo , rather than through expansion of the 1 st CVP . These results support the de novo formation of vessels in the post‐natal heart, which has implications for studies in cardiovascular disease and heart regeneration.