Open AccessEye‐mediated immune tolerance to Type II collagen in arthritis‐prone strains of mice
Author(s) -
Farooq Shukkur M.,
Kumar Ashok,
Ashour Hossam M.
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12376
Subject(s) - collagen induced arthritis , immune system , arthritis , immune tolerance , type ii collagen , immunology , biology , type (biology) , medicine , ecology
Type II collagen (CII) is a cartilage structural protein that plays important roles in joint function, arthritis and ageing. In studying the ability of CII to induce eye‐mediated specific immune tolerance, we have recently proven that CII is capable of inducing anterior chamber‐associated immune deviation ( ACAID ) in Balb/c mice. Here, we study the ability of CII to induce eye‐mediated immune tolerance in strains of mice that are prone to the induction of rheumatoid arthritis. Thus, we hypothesized that CII induces ACAID in DBA /1 mice and in C57 BL /6 mice through the AC route (direct injection) or the intravenous route (adoptive transfer of in vitro ‐generated CII‐specific ACAID macrophages or of CII‐specific in vitro ‐generated T regulatory cells). Specific immune tolerance induction was assessed using both delayed‐type hypersensitivity ( DTH ) and local adoptive transfer ( LAT ) assays. Results indicated the ability of CII to generate CII‐specific ACAID ‐mediated immune tolerance in vivo and in vitro in both DBA /1 mice and C57 BL /6 mice. These findings could be beneficial in studies of immune tolerance induction using CII.