Open AccessFirst evidence of TRPV 5 and TRPV 6 channels in human parathyroid glands: possible involvement in neoplastic transformation
Author(s) -
Giusti Laura,
Cetani Filomena,
Da Valle Ylenia,
Pardi Elena,
Ciregia Federica,
Donadio Elena,
Gargini Claudia,
Piano Ilaria,
Borsari Simona,
Jaber Ali,
Caputo Antonella,
Basolo Fulvio,
Giannaccini Gino,
Marcocci Claudio,
Lucacchini Antonio
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12372
Subject(s) - trpv , trpv6 , transient receptor potential channel , parathyroid adenoma , western blot , medicine , blot , calcium , endocrinology , receptor , chemistry , biology , trpv1 , primary hyperparathyroidism , biochemistry , gene
The parathyroid glands play an overall regulatory role in the systemic calcium ( Ca 2+ ) homeostasis. The purpose of the present study was to demonstrate the presence of the Ca 2+ channels transient receptor potential vanilloid ( TRPV ) 5 and TRPV 6 in human parathyroid glands. Semi‐quantitative and quantitative PCR was carried out to evaluate the presence of TRPV 5 and TRPV 6 m RNA s in sporadic parathyroid adenomas and normal parathyroid glands. Western blot and immunocytochemical assays were used to assess protein expression, cellular localization and time expression in primary cultures from human parathyroid adenoma. TRPV 5 and TRPV 6 transcripts were then identified both in normal and pathological tissues. Predominant immunoreactive bands were detected at 75–80 kD for both vanilloid channels. These channels co‐localized with the calcium‐sensing receptor ( CASR ) on the membrane surface, but immunoreactivity was also detected in the cytosol and around the nuclei. Our data showed that western blotting recorded an increase of protein expression of both channels in adenoma samples compared with normal glands suggesting a potential relation with the cell calcium signalling pathway and the pathological processes of these glands.