Open AccessCharacteristics of circulating CD 31 + cells from patients with coronary artery disease
Author(s) -
Kim Moo Hyun,
Guo Longzhe,
Kim HanSoo,
Kim SungWhan
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12370
Subject(s) - coronary artery disease , chemokine , pathogenesis , flow cytometry , chemotaxis , immunology , stable angina , macrophage , microbiology and biotechnology , medicine , inflammation , biology , pathology , receptor , biochemistry , in vitro
Recently, we reported the properties of CD 31‐expressing cells in healthy individuals. However, the characteristics of CD 31‐expressing cells derived from coronary artery disease ( CAD ) patients remain unknown. This study aimed to investigate the relationship between circulating CD 31 + cells and CAD as well as their biological characteristics. Analysis with flow cytometry revealed that CD 31 + cells (C‐ CD 31) from the peripheral blood ( PB ) of CAD patients exhibited low levels of T‐cell marker and high levels of macrophage marker compared with the PB ‐ CD 31 + cells from healthy individuals (H‐ CD 31). In addition, the expression levels of multiple pro‐angiogenic and chemokine genes were significantly down‐regulated in C‐ CD 31. However, inflammatory gene IL ‐1 α was highly up‐regulated in C‐ CD 31. Patients with unstable angina ( UA ) had significantly more CD 31 + cells in the PB than healthy control group ( P < 0.001). Moreover, there were significant correlations between the number of CD 31 + cells and cardiovascular ( CV ) disease activity ( R = 0.318, P = 0.006) and the number of diseased coronaries ( R = 0.312, P = 0.005). For the diagnostic category of UA , the area under curve was 0.803 ( P < 0.001). In conclusion, C‐ CD 31 have impaired angiogenic potential and the number of circulating CD 31 + cells were correlated with CV risk. These findings may contribute to the understanding of the pathogenesis of CAD .