Open AccessEpigenetic changes of mesenchymal stem cells in three‐dimensional (3D) spheroids
Author(s) -
Guo Ling,
Zhou Ying,
Wang Shan,
Wu Yaojiong
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12336
Subject(s) - spheroid , mesenchymal stem cell , epigenetics , biology , microbiology and biotechnology , stem cell , evolutionary biology , genetics , in vitro , gene
Mesenchymal stem cells ( MSC s) hold profound promise in tissue repair/regeneration. However, MSC s undergo remarkable spontaneous differentiation and aging during monolayer culture expansion. In this study, we found that 2–3 days of three‐dimensional (3D) spheroid culture of human MSC s (h MSC s) that had been expanded in monolayer for six passages increased their clonogenicity and differentiation potency to neuronal cells. Moreover, in accordance with these changes, the expression levels of mi RNA which were involved in stem cell potency were changed and levels of histone H3 acetylation in K9 in promoter regions of Oct4, Sox2 and Nanog were elevated. Our results indicate that spheroid culture increases their multi‐potency and changes the epigenetic status of pluripotent genes in h MSC s.