Open AccessCharacterization of the effect of LPS on dendritic cell subset discrimination in spleen
Author(s) -
Griffiths Kristin L,
Tan Jonathan KH,
O'Neill Helen C
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12332
Subject(s) - lipopolysaccharide , spleen , immunology , dendritic cell , cell , sepsis , antigen , mediator , biology , inflammation , microbiology and biotechnology , biochemistry
The Gram‐negative bacterial endotoxin lipopolysaccharide ( LPS ) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen‐presenting cells, here we find that LPS down‐regulates expression of CD 11c and CD 11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.