Open AccessAdrenaline stimulates the proliferation and migration of mesenchymal stem cells towards the LPS ‐induced lung injury
Author(s) -
Wu Xiaodan,
Wang Zhiming,
Qian Mengjia,
Wang Lingyan,
Bai Chunxue,
Wang Xiangdong
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12283
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , stem cell , lung , chemistry , cancer research , biology , medicine
Bone marrow‐derived mesenchymal stem cells ( BMSC s) could modulate inflammation in experimental lung injury. On the other hand, adrenergic receptor agonists could increase DNA synthesis of stem cells. Therefore, we investigated the therapeutic role of adrenaline‐stimulated BMSC s on lipopolysaccharide ( LPS )‐induced lung injury. BMSC s were cultured with adrenergic receptor agonists or antagonists. Suspensions of lung cells or sliced lung tissue from animals with or without LPS ‐induced injury were co‐cultured with BMSC s. LPS ‐stimulated alveolar macrophages were co‐cultured with BMSC s (with adrenaline stimulation or not) in Transwell for 6 hrs. A preliminary animal experiment was conducted to validate the findings in ex vivo study. We found that adrenaline at 10 μM enhanced proliferation of BMSC s through both α‐ and β‐adrenergic receptors. Adrenaline promoted the migration of BMSC s towards LPS ‐injured lung cells or lung tissue. Adrenaline‐stimulated BMSC s decreased the inflammation of LPS ‐stimulated macrophages, probably through the expression and secretion of several paracrine factors. Adrenaline reduced the extent of injury in LPS ‐injured rats. Our data indicate that adrenaline‐stimulated BMSC s might contribute to the prevention from acute lung injury through the activation of adrenergic receptors, promotion of proliferation and migration towards injured lung, and modulation of inflammation.