Open AccessGr‐1+ CD 11b+ myeloid cells efficiently home to site of injury after intravenous administration and enhance diabetic wound healing by neoangiogenesis
Author(s) -
Tong Xiaozhe,
Lv Gang,
Huang Jianhua,
Min Yongfen,
Yang Li,
Lin Pengnian Charles
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12265
Subject(s) - wound healing , myeloid , homing (biology) , medicine , integrin alpha m , neovascularization , myeloid cells , chemokine , inflammation , pharmacology , immunology , cancer research , angiogenesis , biology , flow cytometry , ecology
Vascularization is an important factor that affects diabetic wound healing. There is increasing evidence that myeloid cell lineages play a role in neovascularization. In this study, the efficiency of Gr‐1+ CD 11b+ myeloid cells to home to the site of injury and enhance diabetic wound healing by neoangiogenesis after intravenous administration was investigated. Gr‐1+ CD 11b+ myeloid cells were injected into tail vein after establishment of dorsal window chamber, hindlimb ischaemia and ear‐punch injury in diabetic or non‐diabetic mice. The Gr‐1+ CD 11b+ myeloid cells efficiently homed to the site of injury after intravenous administration and increased neoangiogenesis. The chemokine receptor type 4 ( CXCR 4) is robustly expressed by Gr‐1+ CD 11b+ myeloid cells. Inhibition of CXCR 4 decreases the homing ability of Gr‐1+ CD 11b+ myeloid cells to the site of injury, which indicates that the CXCR 4/ SDF ‐1 axis plays an important role in the homing of Gr‐1+ CD 11b+ myeloid cells to the site of injury. In addition, Gr‐1+ CD 11b+ myeloid cells were found to improve blood flow recovery of ischaemic limb and enhance wound healing in diabetic mice by neoangiogenesis after intravenous administration. Taken together, the results of this study suggest that Gr‐1+ CD 11b+ myeloid cells may serve as a potential cell therapy for diabetic wound healing.