Open AccessXBP 1S, a BMP 2‐inducible transcription factor, accelerates endochondral bone growth by activating GEP growth factor
Author(s) -
Guo FengJin,
Xiong Zhangyuan,
Han Xiaofeng,
Liu Chuanju,
Liu Yanna,
Jiang Rong,
Zhang Peng
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12261
Subject(s) - endochondral ossification , runx2 , chondrogenesis , chondrocyte , microbiology and biotechnology , bone morphogenetic protein 2 , growth differentiation factor , transcription factor , chemistry , biology , bone morphogenetic protein , mesenchymal stem cell , cartilage , anatomy , biochemistry , in vitro , gene
We previously reported that transcription factor XBP 1S binds to RUNX 2 and enhances chondrocyte hypertrophy through acting as a cofactor of RUNX 2. Herein, we report that XBP 1S is a key downstream molecule of BMP 2 and is required for BMP 2‐mediated chondrocyte differentiation. XBP 1S is up‐regulated during chondrocyte differentiation and demonstrates the temporal and spatial expression pattern during skeletal development. XBP 1S stimulates chondrocyte differentiation from mesenchymal stem cells in vitro and endochondral ossification ex vivo . In addition, XBP 1S activates granulin‐epithelin precursor ( GEP ), a growth factor known to stimulate chondrogenesis, and endogenous GEP is required, at least in part, for XBP 1S‐stimulated chondrocyte hypertrophy, mineralization and endochondral bone formation. Furthermore, XBP 1S enhances GEP ‐stimulated chondrogenesis and endochondral bone formation. Collectively, these findings demonstrate that XBP 1S, a BMP 2‐inducible transcription factor, positively regulates endochondral bone formation by activating GEP chondrogenic growth factor.