Open AccessZinc is essential for the transcription function of Nrf2 in human renal tubule cells in vitro and mouse kidney in vivo under the diabetic condition
Author(s) -
Li Bing,
Cui Wenpeng,
Tan Yi,
Luo Ping,
Chen Qiang,
Zhang Chi,
Qu Wei,
Miao Lining,
Cai Lu
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12239
Subject(s) - endocrinology , medicine , kidney , diabetic nephropathy , transcription factor , oxidative stress , protein kinase b , fibrosis , chemistry , in vivo , fyn , inflammation , renal function , biology , signal transduction , biochemistry , receptor , microbiology and biotechnology , gene , proto oncogene tyrosine protein kinase src
Increasing evidence from human and laboratory studies showed the effect of zinc ( Zn ) on diabetic complications. Nuclear factor‐erythroid 2‐related factor 2 (Nrf2) plays important role in the prevention of oxidative damage. This study was to define whether Zn statues (deficiency or supplement) affect the Nrf2 expression and function, and also affect the damage severity of human renal tubular ( HK 11) cells exposed to high glucose ( HG ) with palmitate (Pal) and kidney of diabetic mice induced by multiple low‐dose streptozotocins. For Zn deficiency diabetic mice were treated with Zn chelator PTEN at 5 mg/kg bw daily for 4 months. Results showed that HG /Pal significantly increased the expression of pro‐fibrotic mediators, connective tissue growth factor and PAI ‐1, in HK 11 cells, which was exacerbated by TPEN that depleted intracellular free Zn and decreased Nrf2 expression and transcription. Zn supplement prevented the effects of TPEN and also increased Akt and GSK ‐3β phosphorylation with a decrease in Nrf2 nuclear exporter, Fyn. All these effects of Zn were abolished by Akt inhibitor. Therefore, Zn up‐regulates Nrf2 function via activating Akt‐mediated inhibition of Fyn function. Treatment of diabetic mice with TPEN decreased renal Zn level and Nrf2 expression and transcription, with an exacerbation of renal oxidative damage, inflammation and fibrosis. These results suggest the essentiality of Zn for Nrf2 expression and transcription function.