Open AccessReprogramming to a pluripotent state modifies mesenchymal stem cell resistance to oxidative stress
Author(s) -
Asensi Karina D.,
Fortunato Rodrigo S.,
Santos Danúbia S.,
Pacheco Thaísa S.,
Rezende Danielle F.,
Rodrigues Deivid C.,
Mesquita Fernanda C. P.,
KasaiBrunswick Tais H.,
Carvalho Antonio C. Campos,
Carvalho Denise P.,
Carvalho Adriana B.,
Goldenberg Regina C. dos S.
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12226
Subject(s) - reprogramming , induced pluripotent stem cell , mesenchymal stem cell , microbiology and biotechnology , oxidative stress , stem cell , embryonic stem cell , reactive oxygen species , biology , catalase , superoxide dismutase , chemistry , cell , biochemistry , gene
Properties of induced pluripotent stem cells (i PSC ) have been extensively studied since their first derivation in 2006. However, the modification in reactive oxygen species ( ROS ) production and detoxification caused by reprogramming still needs to be further elucidated. The objective of this study was to compare the response of i PSC generated from menstrual blood–derived mesenchymal stem cells (mb‐i PSC ), embryonic stem cells (H9) and adult menstrual blood–derived mesenchymal stem cells (mb MSC ) to ROS exposure and investigate the effects of reprogramming on cellular oxidative stress ( OS ). mb MSC were extremely resistant to ROS exposure, however, mb‐i PSC were 10‐fold less resistant to H 2 O 2 , which was very similar to embryonic stem cell sensitivity. Extracellular production of ROS was also similar in mb‐i PSC and H9 and almost threefold lower than in mb MSC . Furthermore, intracellular amounts of ROS were higher in mb‐i PSC and H9 when compared with mb MSC . As the ability to metabolize ROS is related to antioxidant enzymes, we analysed enzyme activities in these cell types. Catalase and superoxide dismutase activities were reduced in mb‐i PSC and H9 when compared with mb MSC . Finally, cell adhesion under OS conditions was impaired in mb‐i PSC when compared with mb MSC , albeit similar to H9. Thus, reprogramming leads to profound modifications in extracellular ROS production accompanied by loss of the ability to handle OS .