Open AccessRegulation of cyp26a1 on Th17 cells in mouse peri‐implantation
Author(s) -
Liu HaiYan,
Chao Huhe,
Liu ZhenKun,
Xia HongFei,
Song Zhihui,
Yang Ying,
Peng JingPian
Publication year - 2014
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12196
Subject(s) - rar related orphan receptor gamma , biology , retinoic acid , antibody , andrology , uterus , flow cytometry , microbiology and biotechnology , spleen , immune system , medicine , endocrinology , immunology , cell culture , foxp3 , genetics
Cytochrome P450 26A1 (cyp26a1) is expressed in the mouse uterus during peri‐implantation. The repression of this protein is closely associated with a reduction in implantation sites, suggesting a specific role for cyp26a1 in pregnancy and prompting questions concerning how a metabolic enzyme can generate this distinct outcome. To explore the effective downstream targets of cyp26a1 and confirm if its role in peri‐implantation depends on its metabolic substrate RA (retinoic acid), we characterized the changes in the peripheral blood, spleen and uterine implantation sites using the cyp26a1 gene vaccine constructed before. Flow cytometry results showed a significant increase in CD 4 + ROR γt + Th17 cells in both the peripheral blood and spleen in the experimental group. The expression of ROR γt and IL ‐17 presented the Th17 cells reduction in uterus followed by the suppression of cyp26a1 expression. For greater certainty, cyp26a1 antibody blocking model and RNA interference model were constructed to determine the precise target immune cell group. High performance liquid chromatography results showed a significant increase in uterine at‐ RA followed by the immunization of cyp26a1 gene vaccine. Both the ascertain by measuring RAR α protein levels in peri‐implantation uterus after gene vaccine immunization and researches using the specific agonist and antagonist against RAR α suggested that RAR α may be the main RA receptor for signal transduction. These results provided more evidence for the signal messenger role of RA in cyp26a1 regulation from the other side. Here, we showed that the cyp26a1‐regulated Th17 cells are dependent on at‐ RA signalling, which is delivered through RAR α in mouse peri‐implantation.